Determination of Amino Acids inQihong Maitong Injection through Pre-column Derivatization HPLC
10.11842/wst.2015.05.030
- VernacularTitle:柱前衍生化HPLC法测定注射用芪红脉通中氨基酸＊
- Author:
Yiwu ZHAO
;
Hongda ZHANG
;
Xue XIE
;
Wei WANG
;
Kui HONG
;
Wenzhe HUANG
;
Zhengzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Qihong Maitong injection;
amino acids;
pre-column determination;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(5):1080-1084
- CountryChina
- Language:Chinese
-
Abstract:
In this article, an HPLC method for the contents determination of amino acids in Qihong Maitong injection was reported. In detailed, OPA-Fmoc pre-column derivatization was adopted, and related HPLC methods to determine the contents of amino acids was established. Linear relationship was well constructed for 17 amino acids through the method mentioned above. Briefly speaking, the optimized method was accurate and reproducible, and suitable for the determination of amino acids in Qihong Maitong injection and corresponding quality control.
