Investigation on Purification Process of Intermediate Product ofCaulis Sinomenii fromTong-An Injection
10.11842/wst.2015.05.032
- VernacularTitle:痛安注射液青风藤中间体纯化工艺研究＊
- Author:
Xiaodong KANG
;
Xufang YANG
;
Feng LI
;
Junchao LIU
;
Yun WU
;
Yongcheng SUN
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Tong-An injection;
Caulis Sinomenii;
transfer rate;
purification process technology
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(5):1090-1094
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to improve the transfer rate of sinomenine in the intermediate product of Caulis Sinomenii in order to optimize the purification process of intermediate product ofCaulis Sinomenii fromTong-An (TA) injection. The transfer rate of sinomenine and the stability of fingerprints in the intermediate product of Caulis Sinomenii were used as indexes for the investigation on the impact from different pH ofCaulis Sinomenii extract before extraction. The transfer rate of sinomenine was used as an index for the investigation on the impact by different pH of hydrochloric acid created to dry extract solution. The transfer rate of sinomenine was used as an index for the investigation of four separation ways, which included the vacuum filtration, plate and frame filters, high-speed tube separator, and flat direct centrifuge, on the liquid separation of sinomenium acutum acid. The results showed that the pH ofCaulis Sinomenii extract before extraction was 10-11; the transfer rate of sinomenine was the highest in the extraction process and the fingerprints of TA injection was stable. The pH of hydrochloric acid was 2.0-2.5; and the highest transfer rate of sinomenine in acid dissolution process was 92.94%. The high-speed tube separator had the best separation to sinomenium acutum acid-dissolving liquid. The highest transfer rate of sinomenine was 93.34%. It was concluded that the optimized process can effectively improve the transfer rate of sinomenine in the intermediate product ofCaulis Sinomenii. Meanwhile, fingerprints of the product were stable. The process was simple with good repeatability.
