Effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.28.003
- VernacularTitle:龙鳖胶囊对骨髓间充质干细胞增殖活性的影响
- Author:
Jianke PAN
;
Baiming GUO
;
Jun LIU
;
Xian ZHANG
;
Hui XIE
;
Da GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(28):4439-4444
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Longbie Capsule has satisfactory outcomes in the treatment of osteoarthritis, but its mechanism is stil unclear.
OBJECTIVE:To study the effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cel s. METHODS:The bone marrow mesenchymal stem cel s from SD rats were separated and expanded by whole adherence culture, then subcultured and confirmed by morphological observation and flow cytometry. Passage 4 cel s were cultured in complete media containing 5 g/L, 1 g/L, 250 mg/L, 50 mg/L, 10 mg/L Longbie Capsule, respectively, for 24 hours. Then, MTT assay was used to detect cel viability.
RESULTS AND CONCLUSION:The primary cel s were adherent cel s characterized by irregular shape, passage 2 cel s were typical y fibrous-shaped, passage 3 cel s grew in long fibrous and swirl-type shape. Passage 4 cel s were strongly positive for CD29 and CD90, positive for CD44, and negative for CD34 and CD45. 5 g/L and 1 g/L Longbie Capsule promoted the proliferation of bone marrow mesenchymal stem cel s. These findings indicate that Longbie Capsule may promote the proliferation and differentiation of bone marrow mesenchymal stem cel s, thereby playing a therapeutic effect on osteoarthritis.
