Vascular endothelial growth factor 165 promotes proliferation of human adipose-derived mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.28.011
- VernacularTitle:血管内皮生长因子165基因促进人脂肪间充质干细胞的增殖
- Author:
Yu WANG
;
Zhitu ZHU
;
Junjiang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(28):4485-4492
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Autologous fat transplantation has been widely used in soft tissue defect repair and cosmetic surgery, and the 1-year transplant survival rate is 20%-80%. Therefore, the establishment of timely and adequate blood supply at early period after transplantation is very important for the survival of transplanted fat tissues.
OBJECTIVE:To observe the proliferation of human adipose-derived mesenchymal stem cel s transfected with vascular endothelial growth factor 165.
METHODS:Human adipose-derived mesenchymal stem cel s were subcultured in vitro. Recombinant adenovirus carrying vascular endothelial growth factor 165 and blank virus were respectively transferred into adipose-derived mesenchymal stem cel s. Cel s cultured normal y served as blank group.
RESULTS AND CONCLUSION:Compared with the control and blank groups, the expressions of vascular endothelial growth factor 165 mRNA and protein were higher in the experimental group (P<0.05). Experimental findings suggest that the recombinant adenovirus carrying vascular endothelial growth factor 165 transferred into adipose-derived mesenchymal stem cel s cannot only maintain the expression of target protein but also obviously promote the proliferation of adipose-derived mesenchymal stem cel s.
