Osteogenesis of adipose stem cells on artificial bone scaffold
10.3969/j.issn.2095-4344.2015.28.012
- VernacularTitle:脂肪干细胞在人工骨支架诱导下的成骨效应
- Author:
Peng SU
;
Weiwei ZHANG
;
Huawei YU
;
Han YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(28):4493-4497
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Insufficient source of seed cel s is the important factor to restrict the tissue reconstruction and the development of regenerative medicine.
OBJECTIVE:To explore the osteogenesis of adipose stem cel s cultured with different kinds of artificial bones.
METHODS:Adipose tissue was extracted from female volunteers undergoing cosmetic surgery to isolate adipose stem cel s. Passage 4 adipose-derived mesenchymal stem cel s were selected and divided into osteogenic induction group, osteogenic induction+hydroxyapatite group, osteogenic induction+absorbable bone group and osteogenic induction+recombinant bone xenograft group. The latter three groups were subdivided into 3, 10, 20 g/L subgroups, respectively. Culture medium was exchanged every 3 days, total y for 12 days.
RESULTS AND CONCLUSION:Compared with the osteogenic induction group, the calcium concentration in the elution liquid was significantly higher in the osteogenic induction+hydroxyapatite group and low-concentration osteogenic induction+absorbable bone group (both P<0.05), but no difference was found between the osteogenic induction group and high-concentration osteogenic induction+absorbable bone group (P<0.05). In addition, the calcium concentration in the elution liquid was significantly lower in the osteogenic induction+recombinant bone xenograft group than the osteogenic induction (P<0.05). Therefore, different artificial bone scaffolds can influence the osteogenic effect of adipose stem cel s, and among them, hydroxyapatite has a better effect on the osteogenic induction of adipose stem cel s.
