Biological characteristics of G666-1 nasopharyngeal cancer stem cells in Survivin gene silencing
10.3969/j.issn.2095-4344.2015.28.015
- VernacularTitle:Survivin基因沉默鼻咽癌G666-1细胞的生物学特性
- Author:
Hui WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(28):4508-4513
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Survivin gene is the most potent anti-apoptosis factor, which can inhibit tumor cel proliferation and migration. It has become a hot topic related to cancer research in recent years.
OBJECTIVE:To explore the effect of Survivin gene silencing on the biological characteristics of G666-1 nasopharyngeal carcinoma cel s.
METHODS:siRNA sequence of Survivin was designed and synthesized and then transfected into G666-1 cel s using LipofectamineTM 2000. After Survivin gene silencing, Survivin mRNA and protein expressions were detected by RT-PCR and western blot assay, respectively. Expression of CD44+CD133+cel s was detected by flow cytometry. In addition, the effects of Survivin gene silencing on the proliferation, migration and invasion of G666-1 cel s were evaluated through cel counting kit-8 test, scratch healing assay and cel invasion assay.
RESULTS AND CONCLUSION:After Survivin gene silencing, the expressions of Survivin mRNA and protein were significantly reduced (P<0.05);the growth of G666-1 cel s was slowed dramatical y, indicating the proliferation ability was inhibited, and the migration and invasion abilities were both decreased significantly (P<0.05);the expression ratio of CD44+CD133+cel s was significantly lowered (P<0.05). These findings indicate that the proliferation, migration and invasion of G666-1 nasopharyngeal cancer stem cel s can be suppressed after Survivin gene silencing, and the proportion of CD44+CD133+cel s is reduced, thus indicating Survivin gene silencing may be a new effective target for treatment of nasopharyngeal carcinoma.
