In vivo homing of bone marrow mesenchymal stem cells transplanted through different ways in rats exposed to silica dust
10.3969/j.issn.2095-4344.2015.28.024
- VernacularTitle:不同方式移植骨髓间充质干细胞在染矽尘大鼠体内的归巢
- Author:
Ming HUANG
;
Yongmei ZHOU
;
Ling YAN
;
Bin LI
;
Qifeng WU
;
Weihui LIANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(28):4562-4566
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone marrow mesenchymal stem cel s transplanted into rats exposed to silica dust can home to the injured lung, but the homing effects via different ways are stil unclear.
OBJECTIVE:To comparatively observe the distribution of bone marrow mesenchymal stem cel s transplanted via different ways into rats exposed to silica dust.
METHODS:Bone marrow mesenchymal stem cel s of donor rats were isolated through whole bone marrow adherent method and transfected by Lv-eGFP. Receptor rats were exposed to silica dust through windpipe injection and randomly divided into intravenous injection and intratracheal injection groups. Then, transfected bone marrow mesenchymal stem cel s were injected via the vein and trachea into acceptor rats. The acceptor rats were kil ed at weeks 1, 2, 3, 4 after transplantation to take the lung, heart, liver, spleen, kidney, and brain tissue that were made into frozen sections and observed under fluorescence microscopy. The intensity of green fluorescence (absorbance value) was analyzed using image analysis software.
RESULTS AND CONCLUSION:Strong, wide and lasting green fluorescence was both observed in the lung tissue of intravenous injection and intratracheal injection groups, which was especial y remarkable around the bronchus and blood vessels. The fluorescence intensities of both two groups were slightly decreased with time, but there was no difference between the two groups (P>0.05). The fluorescence in the other organs of both two groups was also observed at early stage. It was stronger and wider in the liver, spleen and heart, while fainter and less in the kidney and brain, and reduced with time in al the organs. Fluorescence could be observed few and faint only in the liver and spleen at late stage, and could hardly be seen in the brain. The fluorescence intensities of the liver, spleen, heart, kidney and brain had no significant difference between the two groups at the same time (P>0.05), but the fluorescence intensity in the brain at the 1st week showed significant difference between the two groups (P<0.05). These findings indicate that the intravenous injection and intratracheal injection of bone marrow mesenchymal stem cel s have similar homing effects in rats exposed to silica dust.
