Effects of 3-n-butylphthalide pretreatment on microvascular architecture and free radical metabolism in hippocampus induced by cerebral ischemia/reperfusion
10.11958/j.issn.0253-9896.2015.08.010
- VernacularTitle:丁苯酞预处理对脑缺血再灌注大鼠海马微血管构筑和自由基代谢的影响
- Author:
Xiaoying ZHENG
;
Wei KONG
;
Shumin ZHAO
;
Hairu JI
;
Meng CHEN
;
Weijun MA
;
Li HAN
;
Sheng LIU
- Publication Type:Journal Article
- Keywords:
reperfusion injury;
hippocampus;
superoxide dismutase;
malondialdehyde;
3-n-butylphthalide;
microves-sel density;
microvessel area density
- From:
Tianjin Medical Journal
2015;(8):867-870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe microvascular architecture and free radical metabolism in hippocampus after focal cerebral ischemia/reperfusion and to explore the effect of NBP (3-n-butylphthalide). Methods Fifty-four SD rats were ran?domly divided into NBP pretreatment group, ischemia/reperfusion group and sham operation group (n=18 in each group). The model of middle cerebral artery occlusion(MCAO)was established by suture method. The neurological scores were counted and the volume of infarction was measured;TA-Fe method was applied to observe the microvascular architecture of hippo?campus, Mivnt image analysis system was used to analyze the microvessel density(MVD)and the microvessel area density (MVA)of hippocampus quantitatively;The activity of SOD and content of MDA were measured by colorimetric method. Re?sults Compared to the ischemia reperfusion(IR)group, the neurological scores and the volume of infarction were decreased sharply in NBP group. What′s more, the activity of SOD, MVD and MVA were all enhanced but the content of MDA and the count of closed microvessels were both reduced(P < 0.01). Conclusion NBP can improve microvascular architecture of hippocampus and reduce the free radical injury. There is a protective effect on hippocampus of rats who suffered focal cere?bral ischemia reperfusion.
