Research on Content Determination Methods ofTang-Nai-Kang Granules
10.11842/wst.2015.06.009
- VernacularTitle:糖耐康颗粒含量测定方法研究＊
- Author:
Ying WEI
;
Xiaojuan SHI
;
Dongchao WANG
;
Pengshou LI
;
Bo LI
;
Linyi LI
;
Tunhai XU
;
Tonghua LIU
- Publication Type:Journal Article
- Keywords:
Tang-Nai-Kang granules;
rosmarinic acid;
ginsenoside Rg1;
ginsenoside Re;
ginsenoside Rb1;
quality control
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1169-1175
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the content determination methods ofTang-Nai-Kang (TNK) granules. The content of rosemary acid in TNK granules was determined by HPLC method, using C18 chromatographic column (150 mm×4.6 mm, 5μm), the mobile phase of 0.1% trifluoroacetic acid - methanol (60:40),λ of 330 nm. The theoretical plate number was more than 2 000. The standard curve method was used in the determination. Contents of ginsenosides Rg1, Re and Rb1 were determined by HPLC method, using C18 chromatographic column (150 mm× 4.6 mm, 5μm), the mobile phase of water-acetonitrile, gradient elution method,λ of 203 nm. The theoretical plate number was more than 2 000. The standard curve method was used in the determination. The results showed that rosmarinic acid was linear in the range of 0.300-0.500 mg·mL-1 with good linearity. The regression equation wasY = 1E + 07X -7 334,R2 = 0.999. Its sample recovery was 97.32% (RSD 1.25%). Ginsenoside Rg1 was linear in the range of 0.138-0.220 mg·mL-1 with good linearity. The regression equation wasY = 2E + 06X +34 864,R2 = 0. 999. Ginsenoside Re was linear in the range of 0.126-0.250 mg·mL-1 with good linearity. The regression equation wasY = 2E + 06X + 39 879,R2 = 0. 999. Ginsenoside Rb1 was linear in the range of 0.132-0.220 mg·mL-1 with good linearity. The regression equation wasY = 2E + 06X + 39 070,R2 =0. 999. Their sample recoveries were Rg1 97.97% (RSD 1.83%), Re 101.80% (RSD 1.83%), Rb1 98.35% (RSD 1.82%). It was concluded that the content determination method established for the quality control of TNK granules was simple, fast and reliable.
