Study on Synergistic Hypoglycemic ActivityIn Vitroof Three Kinds of Isoflavone in Chickpea and Its Derivatives of Ethoxylation
10.11842/wst.2015.06.010
- VernacularTitle:鹰嘴豆中3种异黄酮及其乙氧基化衍生物体外协同降糖活性研究＊
- Author:
Pengshou LI
;
Ying WEI
;
Xiaojuan SHI
;
Bo LI
;
Dongchao WANG
;
Tunhai XU
;
Tonghua LIU
- Publication Type:Journal Article
- Keywords:
Chickpea;
isoflavones;
structure modification;
HepG2 cell;
hypoglycemic activity
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;(6):1176-1183
- CountryChina
- Language:Chinese
-
Abstract:
Through the ethoxylation structure modification of three isoflavones which were genistein, biochanin A and formononetin in chickpea, the hypoglycemic activity and the synergistic hypoglycemic activity were studied. Ethoxylation structure modification was given on three kinds of isoflavones. The hypoglycemic activity of three kinds of isoflavones and their derivatives were studied. The synergistic hypoglycemic activity of the compounds was also studied. The insulin-resistance HepG2 cell was selected as the model of hypoglycemic activity screening. The results showed that four ethoxylation products were synthesized. And the hypoglycemic activity of genistein was better than biochanin A and formononetin with significant difference (P < 0.05). The maximum dosage of three isoflavones was compared with the positive drug metformin hydrochloride. And the effect was not as good as metformin hydrochloride. There was significant difference (P < 0.05, orP < 0.01). There was no significant difference of effect between the compound a and the compound d (P > 0.05). Effects of the compound b and compound c were not as good as the compound a and compound d. There was statistical difference (P < 0.05). The activity of compound d was significantly improved than the parent compound (P < 0.05). There was no statistical difference on the hypoglycemic activity between combination 5 and metformin hydrochloride (P > 0.05). It was concluded that the combinations of compounds played synergistic effects with better hypoglycemic effect. It provided a basis for developing hypoglycemic agents with the intellectual property rights.
