Correlation between functional status of bone marrow mesenchymal stem cells and disease progression in osteoarthritis patients
10.3969/j.issn.2095-4344.2015.32.009
- VernacularTitle:骨关节炎患者骨髓间充质干细胞功能状态与病情进展的相关性
- Author:
Jian LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(32):5129-5133
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:There is a certain relationship between bone marrow mesenchymal stem cels and articular cartilage, and its celular functional status is one of the causes of osteoarthritis.
OBJECTIVE:To explore the correlation between functional status of bone marrow mesenchymal stem cels and disease progression in patients with osteoarthritis.
METHODS:The clinical data of 45 patients were colected, including 15 cases undergoing arthroscopic knee cruciate ligament reconstruction (control group), 15 cases of mild osteoarthritis (mild osteoarthritis group), 15 cases of severe osteoarthritis (severe osteoarthritis group). Bone marrow mesenchymal stem cels from three groups were culturedin vitro to observe the morphological changes. Proliferative and differentiation ability was detected and compared between the three groups.
RESULTS AND CONCLUSION:At 24 hours of primary culture, the bone marrow mesenchymal stem cels were arranged in regular pattern and spindle-shaped in the three groups with no morphological differences. At 14 days of chondrogenic induction, toluidine blue staining was positive for bone marrow mesenchymal stem cels, but there was stil no difference in the chondrogenic ability in the three groups. Cel counting kit-8 results showed that the proliferative ability of cels was strongest in the control group and weakest in the severe osteoarthritis group. These findings indicate that there is a certain correlation between the functional status of bone marrow mesenchymal stem cels and the progress of the disease.
