Effect of microRNA-7 onαβT lymphocytes of mouse mesenteric lymph nodes
10.3969/j.issn.1000-484X.2015.09.003
- VernacularTitle:microRNA-7对小鼠肠系膜淋巴结αβT淋巴细胞组成的影响
- Author:
Juanjuan ZHAO
;
Shunfei ZHU
;
Hualin XU
;
Yan HU
;
Mengmeng GUO
;
Yijing TAO
;
Ya ZHOU
;
Nalin QIN
;
Jing ZHENG
;
Lin XU
- Publication Type:Journal Article
- Keywords:
miR-7 knockdown;
Mesenteric lymph nodes;
αβT lymphocyte
- From:
Chinese Journal of Immunology
2015;(9):1163-1168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the change of proportions of αβT lymphocytes in mesenteric lymph nodes of miR-7 knockdown (miR-7KD)mice,and preliminarily explore its importance.Methods: The volume,weight index and total cells number of mesenteric lymph node in miR-7KD mice were measured.The pathologic morphology change of mesenteric lymph nodes was observed by HE stai -ning.And the changes on proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice were analyzed by Flow cytometry.Results:Compared with those of WT (wild type) mice,the volume,weight index,and the total cells number of mesenteric lymph node were significantly increased ( P<0.05 ).Moreover ,the pathologic morphology was significantly changed.The proportion and numbers of T lymphocyte were significantly increased;however,the proportion of B lymphocyte were significantly decreased (P<0.05). Notably,the proportion and number of CD4+T cells and CD8+T cells were significantly increased (P<0.05).Meanwhile,CD62L+T cell proportion were vigorously reduced and CD 69+T cell and IFN-γ+T cell proportion were notably up-regulated,which belong to CD4+and CD8+T cell(P<0.05).Conclusion:There was significant influence in the proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice,suggesting that miR-7 might play an important role in the composition and function of lymphocytes in mesenteric lymph nodes.
