EdU-labeled human umbilical cord mesenchymal stem cellsin vitro：5 and 10 μmol/L are the optimal concentrations of EdU
10.3969/j.issn.2095-4344.2015.32.015
- VernacularTitle:以EdU体外标记人脐带间充质干细胞：5，10μmol/L是其最适浓度
- Author:
Wu WANG
;
Fei ZHANG
;
Guicai LI
;
Baojun QI
;
Zhongyan WU
;
Peng CAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(32):5167-5171
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:EdU is a new nuclear marker, and currently, it is rarely reported.
OBJECTIVE: To determine the optimal concentration of EdU to label human umbilical cord mesenchymal stem cels.
METHODS: Human umbilical cord mesenchymal stem cels were isolated, purified and subcultured. Cel morphology and growth were observed under inverted microscope. Flow cytometry was used to identify cel surface markers, as wel as adipogenic identification. EdU at concentrations of 5, 10, 20, 50, 100 μmol/L was used to label human umbilical cord mesenchymal stem cels for 24 hours. The optimal concentration that resulted in the highest labeling efficiency was selected, and then cel proliferation curve was drawn.
RESULTS AND CONCLUSION:Under the inverted microscope, cels grew adherently in a long spindle shape, and EdU-labeled cels had the same morphology. Flow cytometry showed that cels were positive for CD44, and had adipogenic differentiation ability. When the concentration of EdU was 5 and 10 μmol/L, the labeling efficiency was the highest, indicating that 5 and 10 μmol/L are the optimal concentrations of EdU to label human umbilical cord mesenchymal stem cels.
