Effects of Gab2 overexpression on proliferation and migration of human colorectal cancer cell line SW480
10.3969/j.issn.1000-484X.2015.09.013
- VernacularTitle:Gab2过表达对人结直肠癌SW480细胞株增殖和迁移的影响
- Author:
Chenbo DING
;
Jihong FENG
;
Liwen YANG
;
Longmei LI
;
Shanshan LI
;
Chao CHEN
;
Junmin LUO
- Publication Type:Journal Article
- Keywords:
Gab2;
Colorectal cancer;
Lentivirus;
Proliferation;
Migration
- From:
Chinese Journal of Immunology
2015;(9):1210-1213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Gab2 overexpression on the proliferation and migration of human colorectal cancer cell line SW480.Methods: The experimental group (LV-Gab2-GFP group),colorectal cancer SW480 cells were transfected with recombinant lentivirus vector (LV-Gab2-GFP),the negative control group was transfected with negative control lentiviral vector ( LV-GFP) ,and the blank control group without any treatment.The mRNA and protein expression of Gab 2 in cells were identified by RT-PCR and Western blot respectively.Proliferation of the cells was detected by CCK-8 colorimeter and colony forming assay.Wound-healing assay was used to determine the cells migration .Results: RT-PCR and Western blot demonstrated that Gab 2 mRNA and protein expression significantly increased in LV-Gab2-GFP group compared with control groups;overexpression of Gab2 markedly enhanced human colorectal cancer SW 480 cells proliferation and migration compared with control groups .Conclusion:Overexpression of Gab2 accelerates human colorectal cancer SW 480 cells proliferation and migration.
