Stem cells protein microarray analysis after chicken egg-white extract induced cells
10.3969/j.issn.1000-484X.2015.09.015
- VernacularTitle:鸡蛋白提取液诱导细胞后干细胞蛋白芯片分析
- Author:
Guangping RUAN
;
Xiang YAO
;
Jufen LIU
;
Jinxiang WANG
;
Zian LI
;
Yuanyuan HU
;
Xinghua PAN
- Publication Type:Journal Article
- Keywords:
Chicken egg-white extract;
Cells induction;
Stem cell protein chip
- From:
Chinese Journal of Immunology
2015;(9):1217-1220
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the chicken egg-white extracts were co-cultured with cells whether elevated stem cells protein,whether the cells transformation into stem cell.Methods:Four kinds of cells,making a common culture,a 50% chicken egg-white extract co-cultured for 3 days,cells were collected and frozen at -80 degrees,sending the company to do stem cell protein microarray.Results:C57-BMSC has three proteins occurred statistically significant change , TS-UC-MSC has one proteins occurred a statistically significant change ,293T has one protein occurred a statistically significant change ,and 293T-GFP has one protein occurred a statistically significant change.Conclusion:50% chicken egg-white extract co-cultured cells,the cells occurred the phenomenon of transformation into stem cells.
