Effect of miR-199a/b-3p on cell motility of breast cancer cells
10.3969/j.issn.1000-484X.2015.09.021
- VernacularTitle:microRNA-199 a／b-3 p对乳腺癌细胞运动能力的影响
- Author:
Zihang WANG
;
Chunshi LI
;
Jinsong KANG
;
Xuguang MI
;
Lei LIU
- Publication Type:Journal Article
- Keywords:
Breast cancer;
microRNA-199a/b-3p;
Migration and invasion;
Membrane protrusion;
PAK4
- From:
Chinese Journal of Immunology
2015;(9):1242-1244
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the inhibiting mechanism of microRNA-199a/b-3p ( miR-199a/b-3p) on cell motility of breast cancer cells.Methods:The expression of PAK4 in MDA-MB-231 cells transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of MDA-MB-231 cells transfected with miR-199a/b-3p or PAK4 SiRNA were analysed by cell migration assay,invasion assay and protrusion dynamics.Results: MiR-199a/b-3p could suppress the expression of PAK 4 in MDA-MB-231 cells.Comparing with normal control ,miR-199a/b-3p or PAK4 SiRNA could suppress the migration ,invasion and membrane protrusion of MDA-MB-231 cells.Conclusion:miR-199a/b-3p could suppress the expression of PAK4,which are considered key breast cancer suppressors and inhibit the cell motility of breast cancer cells.
