Effect of Acute Hypoxia on Cyclopiazonic Acid Induced Intracellular Calcium Cation Enhancement in Rat Distal Pulmonary Venous Smooth Muscle Cells
10.3969/j.issn.1000-3614.2015.08.020
- VernacularTitle:急性缺氧对环匹阿尼酸诱导的大鼠远端肺静脉平滑肌细胞内钙浓度升高的影响
- Author:
Gongyong PENG
;
Guoping HU
;
Zhuxiang ZHAO
;
Jinxing HU
;
Yimin ZOU
;
Fang PENG
- Publication Type:Journal Article
- Keywords:
Acute hypoxia;
Cyclopiazonic acid;
Pulmonary venous smooth muscle cells;
Intracellular calcium cation concentration;
Store-operated Ca2+ channels
- From:
Chinese Circulation Journal
2015;(8):800-804
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect and the mechanism of acute hypoxia on Ca2+-ATPase inhibitor, cyclopiazonic acid (CPA) induced intracellular calcium cation enhancement in rat distal pulmonary venous smooth muscle cells (PVSMC) .
Methods: The PVSMC were isolated from 6 male SD rats and the cells were cultured for further experiment. Enhancing effects of CPA, acute hypoxia (4% O2) on [Ca2+]i in distal PVSMC and the interventional effects of 2 store-operated Ca2+ channels (SOCC) inhibitors, NiCl2 and SKF96365 on [Ca2+]i in distal PVSMC were tested by lfuorescence microscope and intracellular [Ca2+] examining system.
Results: When PVSMC were perfused with Ca2+-free Krebs solution containing 5 μmol/L nifedipine, 10 μmol/L CPA caused a slight elevation of [Ca2+]i, and acute hypoxia obviously enhanced the [Ca2+]i in PVSMC. When restoration of extracellular [Ca2+] to 2.5 mmol/L, 10 μmol/L CPA caused signiifcant elevation of [Ca2+]i, and acute hypoxia obviously enhanced [Ca2+]i induced by CPA in PVSMC. The SOCC inhibitors, NiCl2 (500 μmol/L) and SKF96365 (50 μmol/L) distinctively attenuated the elevation of [Ca2+]i by hypoxia and CPA. However, NiCl2 and SKF96365 had no effect on high potassium (60 mmol/L KCl Krebs solution) induced elevation of [Ca2+]i in distal PVSMC.
Conclusion: Acute hypoxia enhanced the elevation of [Ca2+]i induced by CPA; such effect could be selectively blocked by SOCC inhibitor which indicated that acute hypoxia could enhance the activity of SOCC in rat distal PVSMC.
