Inhibition effect of interruption PAK4 expression on migration and invasion of hepatocellular carcinoma
10.3969/j.issn.1000-484X.2015.09.007
- VernacularTitle:干扰PAK4表达对肝细胞癌迁移侵袭的影响
- Author:
Zihang WANG
;
Chunshi LI
;
Jinsong KANG
;
Xuguang MI
;
Lei LIU
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
microRNA-199a/b-3p;
Migration;
Invasion;
PAK4
- From:
Chinese Journal of Immunology
2015;(9):1183-1185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effect of PAK4 interruption by microRNA-199a/b-3p (miR-199a/b-3p) on migration and invasion of hepatocellular carcinoma (HCC).Methods: To test targeting of PAK4 by miR-199a/b-3p,we used luciferase assay in HEK293T cells cotransfected miR-199a/b-3p mimcs and pmirGLO-PAK4 3′UTR.The expression of PAK4 in SMMC-7721 transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of SMMC-7721 cells transfected with miR-199a/b-3p or PAK4 Si were analysed by cell migration assay and invasion assay.Results:MiR-199a/b-3p could suppress the mRNA and protein ex-pression of PAK4 by targeting PAK4 3′UTR,and the downregulating PAK 4 expression suppress the migration and invasion of SMMC-7721 cells.Conclusion: MiR-199a/b-3p could suppress the expression of PAK 4, which are considered key HCC suppressors and inhibit the migration and invasion of HCC cells.
