Effect of Shenfu injection on Nrf2 signaling pathway in rats with cerebral ischemia reperfusion-induced brain injury
10.3969/j.issn.1000-484X.2015.09.009
- VernacularTitle:参附注射液对脑缺血再灌注损伤大鼠Nrf2信号通路的影响
- Author:
Chengping JIANG
;
Bihua WU
;
Baiqiang WANG
;
Fei LUO
;
Xiaoping HE
;
Xiaoming WANG
;
Liming LIU
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia reperfusion;
Shenfu injection;
Nrf2;
Rat
- From:
Chinese Journal of Immunology
2015;(9):1191-1194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the neuro-protective effects of Shenfu injection on Nrf 2 signaling pathway affected by cerebral ischemia reperfusion.Methods: A total of 68 adult male Sprague-Dawley rats were randomly divided into sham group , cerebral ischemia-reperfusion group,and(8 mg/kg)Shenfu injection treatment group.Shenfu injection was injected intraperitoneally in the rats after MCAO.Neurologic deficit was evaluated after 24 hours of reperfusion.All the rats were sacrificed after 24 h of ischemia-reoerfusion for biochemical analysis or Nissl staining.Results:Shenfu injection treatment significantly increased the expression of Nrf 2,HO-1 and NQO-1(P<0.05).Furthermore,Shenfu injection treatment significantly reduced the expression of cleaved -caspase-3 and attenuated neurological deficits after cerebral ischemia reperfusion ( P<0.05 ).Lastly, Shenfu injection could evidently alleviate the severity of neuronal degeneration ( P<0.05 ).Conclusion:Shenfu injection could confer neuroprotection after cerebral ischemia reperfusion through modulating the Nrf2 signaling pathway.
