Simultaneous determination of harpagide and harpagoside in Mailuoning injection by HPLC
- VernacularTitle:HPLC法同时测定脉络宁注射液中哈巴苷和哈巴俄苷的含量
- Author:
Changkai ZHOU
;
Jing GAO
;
Shiqiang SHI
;
Liping HAO
;
Fanbo JING
- Publication Type:Journal Article
- Keywords:
Mailuoning injection;
harpagide;
harpagoside;
content determination;
HPLC
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(8):173-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for content determination of harpagide and harpagoside in Mailuoning injection by HPLC. Methods The experimental condition of HPLC method was as follows: SunfireTM C18 column (4.6 mm ×150 mm, 5 μm), with gradient elution using acetonitrile and 0.03% phosphoric acid; the detected wavelength was 210 nm, and the flow rate was 1.0mL/min.ResuIts Harpagide and harpagoside demonstrated good linear relationship in the range 0.1424~0.8544 μg/mL(r=0.9998) and 0.0732~0.4392μg/mL (r=0.9997) respectively.The average recovery rate were 98.22% and 99.27% with RSD of 1.46% and 1.42%(n=6)respectively.ConcIusion The method is simple, reliable, accurate, reproducible and stable, and it could be used in the determination of harpagide and harpagoside in Mailuoning injection.
