Effects of Gross Saponins of Tribulus terrestris L. on Inflammatory Reaction and Permeability of Blood-brain Barrier in Rats Following Cerebral Ischemic Injury
10.3870/j.issn.1004-0781.2015.09.002
- VernacularTitle:蒺藜皂苷对局灶性脑缺血大鼠炎症反应和血脑屏障通透性的影响?
- Author:
Fengguo ZHAI
;
Fubo ZHOU
;
Houzhong LI
;
Sufen GUO
;
Feng LIN
;
Lixin GUAN
- Publication Type:Journal Article
- Keywords:
Gross saponins of Tribulus terrestris L.;
Ischemia-reperfusion,cerebral;
Inflammation;
Blood-brain barrier;
Matrix metalloproteinase
- From:
Herald of Medicine
2015;(9):1131-1134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of gross saponins of Tribulus terrestris L.on inflammatory reaction and permeability of blood-brain barrier in rats following cerebral ischemia-reperfusion injury and their potential mechanisms. Methods Sixty SD rats were divided into sham operation group,model control group,gross saponins of Tribulus terrestris L.at low-dose (10 mg?kg-1 )and high-dose groups(30 mg?kg-1 ).Cerebral ischemia -reperfusion model was established with suture emboli method in middle cerebral artery of rats.Neural injury scores,the contents of Evans blue ( EB) and myeloperoxidase( MPO) activities in rat brain were measured 24 hours after the cerebral reperfusion post 2 h ischemia.Content of tumor necrosis factor-α (TNF-α) in rat brain was detected by ELISA; expression levels of matrix metalloproteinase-9(MMP-9) in rat brain was determined by Western blot. Results Compared to the model control group,the neurological deficit scores were significantly decreased(P<0.05),MPO activities and EB contents decreased(P< 0.05 or P< 0.01) in the treatment groups.The expression levels of TNF-α were significantly lower in the treatment groups(0.760±0.110) mg?g-1 and (0.670±0.073) mg?g-1 compared to (0.920±0.128) mg?g-1 in the model control group ( P< 0.05 or P< 0.01). The MMP-9 expression levels were (1.770± 0.181)% and(1.480±0.146)%,significantly lower than(2.200±0.186)% in the model control group(P<0.01). Conclusion Gross saponins of Tribulus terrestris L. exert neuroprotective effects on cerebral ischemia-reperfusion injury in rats through inhibiting the inflammatory reaction and decreasing the permeability of blood-brain barrier,which may be associated with the decrease of the TNF-α content and downregulation of the MMP-9 expression.
