Protective effect of tissue kallikrein against inflammation following focal cerebral ischemia-reperfusion in diabetic rats
10.16571/j.cnki.1008-8199.2015.09.002
- VernacularTitle:组织激肽释放酶对糖尿病大鼠局灶性脑缺血再灌注后炎症损伤的干预作用
- Author:
Ruifeng SHI
;
Ling LIU
;
Bin HU
;
Xin CHEN
;
Qinqin CAO
;
Lingling ZHAO
;
Renliang ZHANG
- Publication Type:Journal Article
- Keywords:
Tissue kallikrein;
Diabetes mellitus;
Cerebral ischemia-reperfusion;
Microglial cell;
Neutrophil;
Intercellular adhesion molecule 1;
Vascular cell adhesion molecule 1
- From:
Journal of Medical Postgraduates
2015;(9):904-909
- CountryChina
- Language:Chinese
-
Abstract:
Objective It remains to be confirmed whether tissue kallikrein has neuroprotective effect in diabetes-induced stroke.This study was to investigate the neuroprotection of tissue kallikrein against cerebral ischemia-reperfusion injury in diabetic rats. Methods Healthy male SD rats were randomly divided into a sham operation, a saline control, and a tissue kallikrein group.Diabetes mellitus was induced in the animals by intraperitoneal injection of streptozotocin and the model of focal cerebral ischemia-reperfusion was made with an intraluminal vascular occlusion method. At 24 hours after modeling, we obtained the neurological deficit score, in-farct size, and brain water content, counted Iba1-and MPO-positive cells by immunohistochemistry, and determined the expressions of ICAM-1 and VCAM-1 by real-time PCR. Results In comparison with the saline controls, the rats treated with tissue kallirein showed significant decreases in the neurological deficit score (P<0.01), the infarct size ([23.57 ±5.79] vs [47.97 ±1.19]%, P<0.01), brain edema ([81.73 ±2.10] vs [84.94 ±2.34]%, P<0.05), the counts of Iba1-and MPO-positive cells (12.33 ±4.46 vs 31.83 ±8.13 and 13.83 ±4.49 vs 37.50 ±7.64, both P<0.01), and the expressions of ICAM-1 and VCAM-1 (both P<0.05). Conclusion Tissue kallikrein has a neuroprotective effect against cerebral ischemia-reperfusion injury in diabetic rats, which may be associated with its anti-inflammation property.
