Protective Effect of Total Glucosides of Mudan Cortex on Acute Myocardial Ischemia in Mice
10.3870/j.issn.1004-0781.2015.09.009
- VernacularTitle:丹皮总苷对急性心肌缺血损伤小鼠的保护作用?
- Author:
Xiaoyan XU
;
Ying LI
;
Wei WANG
;
Hao WANG
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
Total glucosides of Mudan cortex(TGM);
Ischemia,myocardial,acute;
Protective effect
- From:
Herald of Medicine
2015;(9):1158-1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of total glucosides of Mudan cortex(TGM) on acute myocardial ischemia in mice and its mechanism. Methods The total of 60 mice were randomly divided into 5 groups (n = 12),normal control and model control (were given equal capacity of 0.9% sodium chloride solution),and TGM at low,middle,high dose (were given with 50,100,200 mg?kg-1 TGM).The mice were administered once daily for consecutive seven days.After the last administration,the mice in the model control and drug groups were treated by intraperitoneal injection of 15 mg ? kg-1 isoproterenol to to make myocardial ischemia animal model. TGM on the T wave and J point on ECG, and serum lactate dehydrogenase (LDH),myocardial tissue superoxide dismutase (SOD),malondialdehyde (MDA) changes was detected,and the extent of myocardial ischemic injury in mice was measured by Nagar-Olsen staining. Results TGM significantly reduced the displacement of ECG T wave and J point,and improved the related biochemical indexes in mice with myocardial ischemia.The activity of LDH [(898.992± 285.108) μmol?mg-1 ] ,the content of MDA [(11.737 ±5.162) nmol?mg-1 ]in mice treated with TGM at high dose obviously decreased in comparison to the model controls,and the activity of myocardial SOD [(45. 505 ± 20.711) U?mg-1 ] significantly elevated compared with the model control.It was showed that TGM significantly diminished the areas of cardiac muscles ischemia injured via Nagar-Olsen staining. Conclusion TGM has the remarkable protective effect on acute myocardial ischemia injury in mice.
