GDNF regulates the proliferation of glioma cells through AKT/β-catenin signaling pathway
10.3969/j.issn.1671-8348.2015.22.003
- VernacularTitle:GDNF 通过 AKT/β-catenin 调节人胶质瘤细胞增殖的研究
- Author:
Zhouru LI
;
Daohui TENG
;
Guokai DONG
;
Wenjiang YIN
;
Hongxing CAI
- Publication Type:Journal Article
- Keywords:
glioma;
glial cell line-derived neurotrophic factor;
AKT;
β-catenin
- From:
Chongqing Medicine
2015;(22):3034-3036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism that glial cell line-derived neurotrophic factor (GDNF)promotes human glio-ma cells proliferation.Methods We divided glioma samples into two groups,including low-grade glioma group and high-grade glio-ma group,while cerebral contusion patients were treated as the control group,12 cases in each group.C6 glioma cell lines were di-vided into three groups,such as GDNF group,BSA(bovine serum albumin)group and control group.CCK-8 (cell counting kit-8) was used to detect the cell proliferation,while Western blot was used to detect the expression of AKT,p-AKT,β-catenin and p-β-catenin in each group.Results Comparing with the control group,the expression levels of AKT,p-AKT,β-catenin and p-β-catenin in glioma group had a significantly increased (P <0.05).Meanwhile,the high-grade gliomas group also had a significant increase in those more than low-grade gliomas group (P <0.05).CCK-8 test showed that the cell proliferation in GDNF group was significant-ly higher than the control group (P <0.05),and the expression levels of p-AKT,β-catenin and p-β-catenin proteins all had a signifi-cant increase (P <0.05).However,the expression level of AKT had no obvious difference.Conclusion GDNF might promote the proliferation of glioma cells by up-regulating the expression of p-AKT,β-catenin and p-β-catenin.
