The effect of different staining reagent and time on acid fast bacilli staining and its quality control significance
10.3969/j.issn.1671-8348.2015.22.015
- VernacularTitle:不同染色试剂和时间对组织抗酸杆菌染色的影响及其质量控制的意义
- Author:
Jin LIU
;
Gang LI
;
Huaizhi YUAN
- Publication Type:Journal Article
- Keywords:
indicators and reagents;
staining and labeling;
organization of acid fast bacilli;
quality control
- From:
Chongqing Medicine
2015;(22):3066-3067
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of different staining reagent and time on acid fast bacilli staining and study its quality control measures.Methods We collected 38 cases for positive acid fast bacilli stain.Every wax blocked into 4 pieces,with different staining reagent and time of acid fast staining.Results Using xylene and ethanol staining for 20 minutes,acid fast bacilli was discontinuous and red punctate.Zero cases were positive and the positive rate was 0%.Turpentine dyeing for 1 5 minutes group,acid fast bacilli was clear,bright red,slightly bent branched,and easy to identify.A total of 34 cases were positive and posi-tive rate was 89%.Turpentine dyeing process for 20 minutes,the whole background was red,and was not easy identification.A total of 28 cases were positive and the positive rate was 74%.Turpentine dyeing for 30 minutes,the background was entirely deep red, and was hard to discern the acid fast bacilli.A total of 27 cases were positive and the positive rate was 71%.Conclusion Different staining reagent and time had different positive staining results.Suitable turpentine process was stained for 1 5 minutes for acid fast bacilli.
