The effect of monoclonal antibody coated with anti-human CD3 and CD28 on CIK cells growth

VernacularTitle:不同组合 CD3、CD28抗体对 CIK 细胞诱导培养的探索研究
Author: Zhiya SUN ; Jing HE ; Minjia CHEN ; Zonglin ZHAO
Publication Type:Journal Article
Keywords: flow cytometry; CIK cell; immobilization on solid phase materials
From: Chongqing Medicine 2015;(22):3096-3098
CountryChina
Language:Chinese
Abstract: Objective To explore the enhancing effect of monoclonal antibody coated with anti-human CD3 and CD28 on acti-vation and transformation of peripheral blood mononuclear cell (PBMC)in vitro.Methods Human peripheral blood mononuclear cells were separated.Cells was cultured in vitro,and determined by flow cytometry.The solid phase with CD3 and CD28 antibody was coated and added in.The mature CIK cells were obtained after 12 days culturing.Results The CD4 + cells was lower in group C than those in group A(P <0.05).The CD8 + cells was higher in group C than that in group A and B(P <0.05).There was signifi-cant difference of T4/T8 between group C and group A and B(P <0.05 ).There was significant difference of NK cells between group B and group C(P <0.05).The CD25 + cells was lower in group C than that in group A (P <0.01).Conclusion CD3 antibody solid coated combined with CD28 antibody added to the suspension has more strong activation than both CD3 antibody and CD28 antibody solid coating on peripheral blood mononuclear cell.