Polyvinyl alcohol/hydroxyapatite composite hydrogel for the repair of articular cartilage defects: a histocompatibility study
10.3969/j.issn.2095-4344.2015.30.015
- VernacularTitle:聚乙烯醇/羟基磷灰石复合水凝胶修复膝关节软骨缺损的组织相容性
- Author:
Zhijian LEI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(30):4832-4836
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:Hydroxyapatite and polyvinyl alcohol composite hydrogel used for the repair of cartilage defects can have good biological activity in the cartilage joints, effectively promote the growth of bone cels, and enhance the stability and biological activity of implant materials. OBJECTIVE:To explore the histocompatibility of polyvinyl alcohol/hydroxyapatite composite hydrogel transplantation for the repair of articular cartilage defects. METHODS: Twenty New Zealand rabbits were enroled and randomly divided into control group (n=6) and experimental group (n=14). Unilateral knee cartilage defect models were constructed in the two groups. No treatment was done in the control group, and polyvinyl alcohol/hydroxyapatite composite hydrogel transplantation was performed in the experimental group. After 4, 8, 12 weeks, the animals were kiled to colect knee joint specimens for gross observation and histological observation. RESULTS AND CONCLUSION:The surface of articular cartilage in the control group was not recovered at 12 weeks after operation, and granulation tissues were ful of cartilage defects. In the experimental group, the polyvinyl alcohol/hydroxyapatite composite hydrogel was filed in the defects, and tightly connected with the surrounding cartilage tissues with clear boundary and no cel ingrowth at 4 weeks after operation. Until the 12th week, in the experimental group, the composite hydrogel was white and translucent with smooth surface. There was a clear boundary between the composite hydrogel and surrounding cartilage tissues, a great amount of cartilage cels proliferated in the interface that tightly bound to the surrounding tissues, and osteoid tissue grew. These findings indicate that the polyvinyl alcohol/hydroxyapatite composite hydrogel has good histocompatibility in the repair of cartilage defects of rabbit knee joints.
