Study On Dynamic Changes of Ghrelin of Spleen-qi-deficiency Rats and Intervention Effects ofSijunzi Decoction
10.3969/j.issn.1005-5304.2015.09.019
- VernacularTitle:脾气虚证大鼠脑肠肽动态变化及四君子汤干预效应研究
- Author:
Rong TIAN
;
Zihan GONG
;
Xiaoyi YANG
;
Liming ZHU
;
Yongqiang DUAN
;
Yingxia CHENG
;
Juan DU
;
Lanzhen LI
;
Yan WANG
- Publication Type:Journal Article
- Keywords:
spleen qi deficiency;
Sijunzi Decoction;
gastrin;
motilin;
somatostatin;
vasoactive intestinal peptide;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(9):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of ghrelin of spleen-qi-deficiency rats and the intervention effects ofSijunzi Decoction.Methods Experimental rats were randomly divided into normal control group, model group andSijunzi Decoction group. Except for normal control group, spleen-qi-deficiency model was copied through the two-factor methods of breaking qi by bitter cold and swimming exhausted. Meanwhile,Sijunzi Decoction group was given 20 g/(kg?d)Sijunzi Decoction intervention. The activities of GAS, MTL, SS and VIP at different time points (14, 21, 28 d) in intestine and serum were detected by ELISA and RIA. At the same time the intervention effect of Sijunzi Decoction was studied.Results Compared with normal control group, GAS and MTL in intestine and serum of model rats decreased, while SS and VIP increased (P<0.05,P<0.01). Compared with model group, GAS and MTL in intestine and serum of rats in theSijunzi Decoction group increased, while SS and VIP decreased (P<0.05,P<0.01).Conclusion Ghrelin in intestine and serum of spleen-qi-deficiency rats shows dynamic coincidental changes.Sijunzi Decoction can treat spleen qi deficiency by regulating the activities of rat ghrelin.
