Binding capacity of nano-hydroxyapatite/polyamide composite material on the interface of artificial humeral head
10.3969/j.issn.2095-4344.2015.30.002
- VernacularTitle:纳米羟基磷灰石聚酰胺复合材料在人工肱骨头柄界面的结合能力
- Author:
Yong LIU
;
Wei HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(30):4764-4768
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Nano-hydroxyapatite/polyamide 66 is a new nanobionic composite material and has good biocompatibility and bone conduction, but after implantation, whether the composite material can bind to vertebral bone interface and whether it is safe in clinical use stil need further studies. OBJECTIVE: To observe the ability of nano-hydroxyapatite/polyamide composite binding to the interface of artificial humeral head. METHODS: The rabbits were randomly divided into two groups: composite group was implanted with nano-hydroxyapatite/polyamide composites; control group implanted with autologous bone. After the replacement, rabbit bone marrow mesenchymal stem cels were subjected to primary culture, and then the cels were co-cultured with the composite material. RESULTS AND CONCLUSION:At 24 hours after culture, bone marrow mesenchymal stem cels grew wel on the composite materials. The cel adhesion rate was significantly higher in the composite group than the control group at 1 and 4 hours after culture (P < 0.05). After co-culture 4.5, 5.5, 6.5 hours, the absorbance value of cels in the composite group was higher than that in the control group (P < 0.05). At 24 weeks after replacement, changing ranges in the proportions of bone tissue and fibrous layers on the implant surface as wel as coverage rate in the composite group were the highest (P < 0.05). These findings indicate that the nano-hydroxyapatite/ polyamide composite implant has a certain binding capacity, which can make a good interface osseointegration.
