Effects of Simplified Recipe ofBuyang Huanwu Decoction on Expression of APJ in Brain of Focal Cerebral Ischemia Rats
10.3969/j.issn.1005-5304.2015.09.014
- VernacularTitle:补阳还五精简方对局灶性脑缺血大鼠脑组织 APJ表达的影响
- Author:
Feng LI
;
Yuhong WANG
;
Yan SHE
;
Jian YI
;
Xiangyi XIA
;
Hu TAN
;
Le SHAO
;
Guangxian CAI
- Publication Type:Journal Article
- Keywords:
simplified recipe ofBuyang HuanwuDecoction;
focal cerebral ischemia;
APJ;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(9):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of simplified recipe ofBuyang Huanwu Decoction on expression of APJ in the brain of local cerebral ischemia rats;To discuss its mechanism of action. MethodsFocal cerebral ischemia rat models were established by middle cerebral arterial occlusion. The adult rats were randomly divided into sham-operation group, model group,Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group. Administration groups were given relevant medicine for gavage. The expressions of APJ protein and APJ mRNA at different time points were detected by Western blot and RT-PCR.ResultsCompared with model group and sham-operation group, the expression of APJ protein and APJ mRNA at different time points in Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group significantly increased (P<0.05). The expression of APJ protein at different time points showed no difference between simplified recipe ofBuyang Huanwu Decoction group andBuyang Huanwu Decoction group;while the expression of APJ mRNA in simplified recipe ofBuyang Huanwu Decoction group was higher than Buyang Huanwu Decoction group (P<0.05).ConclusionSimplified recipe ofBuyang Huanwu Decoction plays a role in neural protection and restoration by promoting the expression of APJ in the brain of focal cerebral ischemia rats.
