Study on Neurobehavioral Disorders and NMDA Receptor Expression in Rats with Kidney-deficiency Constitution
10.3969/j.issn.1005-5304.2015.09.015
- VernacularTitle:复合型肾虚质大鼠神经行为障碍与NMDA受体表达研究
- Author:
Yuru SUN
;
Yaoguang SUN
;
Qi ZHANG
;
Xiaodi WANG
;
Xing WANG
;
Lijun SUN
- Publication Type:Journal Article
- Keywords:
kidney-deficiency constitution;
suspension test;
Y maze test;
NR2A;
NR2B;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(9):52-55
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo discuss the correlation between kidney-deficiency constitution and brain function decline, and material basis of kidney-storing-spirit theory and neurobiology mechanism. Methods By employing the method of “cats scare rats”, composite offspring rat models with deficiency and acquired dystrophy were built, then they were divided into model group,ZuoguiPill group and Yougui Pill group. Blank group was composed from normal pregnant rats. When the intimidation of offspring began,Zuogui Pill andYougui Pill groups received gavage with corresponding doses of medicine, 1 times per day for 2 consecutive months. Model and blank groups received the same amount of normal saline. Exercise capacity was detected by suspension test. Learning and memory capacity was detected by Y maze test. Expressions of NMDA receptor subunits NR2A and NR2B were detected by immunohistochemical method.Results In suspension test, the duration of model group was shorter than blank group (P<0.05), while duration ofZuogui Pill andYougui Pill groups was longer than the model group (P<0.05). In Y maze test, the correct number of model group was less than blank group, and increased significantly after treatment (P<0.05). The optical density of NR2A and NR2B was lower in model group than blank group (P<0.05) and higher inZuogui Pill andYougui Pill groups than model group (P<0.05).ConclusionThe neurobehavioral is abnormal and NMDA receptor expression depresses in rats with kidney- deficiency constitution.ZuoguiPill andYouguiPill can rise NMDA receptor expression and improve brain function of rats, which reveal the correlation between kidney-deficiency constitution and brain function decline, and material basis of kidney-storing-spirit theory and neurobiology mechanism.
