High-resolution melting analysis for detection of K-ras mutation in colorectal cancer and its clinical significance
10.16571/j.cnki.1008-8199.2015.08.013
- VernacularTitle:高分辨率熔解曲线分析法检测结直肠癌组织 K-ras 基因突变及其临床意义
- Author:
Qian WANG
;
Ye GU
;
Jie LIN
- Publication Type:Journal Article
- Keywords:
High-resolution melting analysis;
Colorectal cancer;
K-ras gene;
Mutation
- From:
Journal of Medical Postgraduates
2015;(8):840-842
- CountryChina
- Language:Chinese
-
Abstract:
Objective The K-ras gene plays a major role in the development , progression, and drug selection for the target treatment of colorectal cancer .The aim of this study was to evaluate the feasibility of detecting K-ras mutation in colorectal cancer by high-resolution melting ( HRM) analysis and to investigate the relationship between K-ras mutation and the clinicopathological parame-ters of colorectal cancer. Methods We collected the tissue samples of colorectal cancer from 179 patients, detected the mutations in codons 12 and 13 of the K-ras gene, and analyzed the relationship between K-ras mutation and the clinicopathological parameters of the patients. Results In the 179 cases of colorectal cancer, K-ras mutation was found in 77 (43.02%), significantly higher in those aged ≥60 years than in those aged <60 years (55.17% vs 33.70%, P<0.05).Multivariate logistic regression analysis showed a significant influence of age on K-ras mutation (OR=1.506, 95%CI:1.028-2.011, P<0.05). Conclusion HRM analysis is a rapid, sensitive, and inexpensive diagnostic tool for the detection of K-ras mutation, and K-ras mutation is associated with the age of colorectal cancer patients .
