Effect of Electroacupuncture on Striatal D1R and DAT Expressions in Cerebral Ischemia-reperfusion Rats
10.13460/j.issn.1005-0957.2015.08.0791
- VernacularTitle:电针对脑缺血再灌注大鼠纹状体D1R和DAT表达的影响
- Author:
Mingshu XU
;
Chunyan CHEN
;
Changzhi LI
;
Linbao GE
;
Shujing ZHANG
;
Dan ZHAO
;
Mingzhe LI
;
Qing HAN
;
Yingjie ZHANG
- Publication Type:Journal Article
- Keywords:
Electroacupuncture;
Cerebral ischemia-reperfusion;
Dopamine D1 receptor;
Dopamine transporter;
Immunohisto-chemistry;
Rats
- From:
Shanghai Journal of Acupuncture and Moxibustion
2015;(8):791-793
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of dopamine D1 receptor (D1R) tool drugs and combined acupuncture and medicine on striatal expressions of D1R and dopamine transporters (DAT) in middle cerebral artery occlusion (MCAO)-reperfusion rats.MethodForty-seven male SD rats were randomly grouped, used to make a model and given corresponding interventions. The materials were taken and fixed six hrs later. Striatal D1R and DAT expressions were detectedby an immunohistochemical method in different groups.ResultThe neurological deficit score was significantly higher in the model group than in the blank group. Electroacupuncture treatment decreased the score significantly (P<0.05). D1R expression was significantly down-regulated in the antagonist, electroacupuncture and electroacupuncture plus antagonist groups compared with the model group (P<0.05). There was no statistically significant difference in D1R expression between the model group and the antagonist or electroacupuncture plus antagonist group (P>0.05). DAT expression was significantly down-regulated in the other groups compared with the model group (P<0.05). There was no statistically significant difference in D1R expression between all groups except the model group (P>0.05).ConclusionCerebral ischemia-reperfusion can result in high D1R and DAT expressions in rat striatum on the ischemic side. Electroacupuncture, D1R antagonists and a combination of the two can significantly down-regulate D1R expression and have a protective effect on the brain. The effects of electroacupuncture and D1R antagonists can not be added to each other. D1R signaling pathway may be one of ways by which electroacupuncture produces a protective effecton the brain.
