Clinical Significance of Detection Antibody of Liver Antigens in Patients with Autoimmune Hepatic Diseases and Viral Hepatitis
10.3969/j.issn.1671-7414.2015.03.047
- VernacularTitle:自身免疫性肝病和病毒性肝炎患者血清中抗肝抗体检测的临床意义
- Author:
Jiang LIN
;
Ke WANG
;
Sunyun CHEN
- Publication Type:Journal Article
- Keywords:
autoimmune liver disease;
immune-blotting assay;
autoantibody
- From:
Journal of Modern Laboratory Medicine
2015;(3):153-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the positive incidence and clinical significance of auto-antibody of liver antigens in the serum of patients with autoimmune hepatitis diseases and viral hepatitis.Methods The serum samples with hepatic diseases were col-lected in Jiangyin People’s Hospital from 2010 to 2014 year.Patients were divided into three groups according to diseases:autoimmune hepatic disease group including autoimmune hepatitis (AIH)12 cases and primary biliary cirrhosis (PBC)36 case;viral hepatic group including HAV 23 cases,HBV 30 cases,HCV 14 cases and HEV 8 cases;normal control group 30 cases.Auto-antibody of live antigens AMA-M2,LKM-1,LC-1,SLA/LP,GP210 and SP100 were tested respectively by west-ern blotting assay.Results The positive rates of anti-AMA-M2,anti-LKM-1,anti-LC-1,anti-SLA/LP,anti-GP210,anti-SP100 were 16.7%,16.7%,8.3%,25%,0% and 16% respectively in AIH group,83.3%,0%,0%,0%,44.4% and 27.8% respectively in PBC group.Patients with viral hepatitis,one case were anti-AMA-M2 positive,one case were anti-GP210 positive and three case with positive anti-SP100.anti-AMA-M2,anti-GP210 and anti-SP100 were detected more fre-quently in autoimmune liver diseases group than viral hepatitis group (χ2 = 33.9,10.6 and 8.8,P < 0.05),anti-SLA/LP were detected more frequently in AIH group than PBC group (χ2 = 6.4,P <0.05),while anti-AMA-M2,anti-GP210 and were detected more frequently in PBC group than AIH group (χ2 =15.1 and 6.1,P <0.05).The positive rate of viral hepat-ic group was a very low frequency,there are little statistically significant difference (χ2 =1.1,P >0.05)compared with nor-mal control group.Conclusion The antibody of liver antigens are useful clinically for diagnosing and classifying autoimmune liver disease.
