Research on Detecting Doubling Time of Human and Rat Airway Smooth Muscle Cells with A Real Time Cellular Analyzer
10.3969/j.issn.1671-7414.2015.03.008
- VernacularTitle:利用实时细胞分析系统检测人和大鼠气道平滑肌细胞倍增时间的研究
- Author:
Wenqian WANG
;
Yu WANG
;
Yudong XU
;
Yongqing YANG
;
Leimiao YIN
- Publication Type:Journal Article
- Keywords:
doubling time;
airway smooth muscle cells;
real time cellular analysis
- From:
Journal of Modern Laboratory Medicine
2015;(3):28-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the doubling time of airway smooth muscle cells of human and rat by the xCELLigence in-strument,a real time cellular analyzer.Methods The airway smooth muscle cells were separated by collagenase-pancreatin digestion from the rat airway.Then,added to the different holes of E-plate of xCELLigence instrument with the planting destiny of 3 000 cells/well.and so were the human airway smooth muscle cells.The E-plate was then placed on the xCELLi-gence instrument to monitor cell proliferation for 100 hours to calculate the doubling time by using the RTCA Software Package 2.0 software.Results The doubling time of human airway smooth muscle cell calculated by the real time cellular analyzer was 23.96±0.47 h,which was consistent with the data provided by the reference.The doubling time of rat airway smooth muscle cell was 18.62±0.15 h,and the computational process was simple,time-saving and also effective.Conclusion The xCELLigence instrument can be used to calculate doubling time of airway smooth muscle cells of human and rat, which provides experimental methods and reference data for the basic respiratory disease research.
