Study on the Method of 0·9 g/dl Normal Saline Dilution to Solve EDTA Dependent Pseudothrombocytopenia
10.3969/j.issn.1671-7414.2015.02.040
- VernacularTitle:0·9 g／dl 生理盐水稀释解决EDTA 依赖性血小板假性减少的方法研究
- Author:
Aisheng LIU
;
Yan WEN
- Publication Type:Journal Article
- Keywords:
0.9 g/dl NaCl solution;
diluting instrument method;
ethylenediamine tetraacetic acid dipotassium dependent plate-let pseudo reduce disease
- From:
Journal of Modern Laboratory Medicine
2015;(2):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 0.9 g/dl NaCl diluting instrument method to solve the ethylenediamine tet-raacetic acid dipotassium (EDTA)anticoagulant dependency pseudo reduce platelet syndrome (PTCP)feasibility,provides solutions to clinical laboratory PTCP more effective method.Methods From August to October of 2014 in their laboratory for PTCP cases in all 3 cases,2 ml venous blood in EDTA and citron acid sodium anticoagulation in-line blending,in the im-mediate,10,30,40 and 60 min computer detection.Collected of peripheral blood in blood thinners,respectively,0.9 g/dl NaCl solution blending,in the immediate,10,30,40 and 60 min computer detection,and compared with the manual method of ammonium oxalate.Results EDTA,citron acid sodium,blood thinners and 0.9 g/dl NaCl diluting instrument immediately detected PTCP blood PLT result compared with ammonium oxalate method,there were no statistically significant difference (t=0.943~1.537,P >0.05),10 min~60 min anticoagulant blood PLT results significantly decreased,compared with am-monium oxalate method difference had statistical significance (t = 12.413 ~ 12.413,P <0.01 ~0.001).Citron acid sodium PLT began to decline after 30 min,compared with ammonium oxalate method,the difference was statistically significant (t=4.915~4.915,P <0.05~0.01).Blood dilution method in 30~40 min PLT test results began to decline,but not obvious, there was no statistically significant difference with the method of ammonium oxalate (t=1.315~1.715,P >0.05),40~60 min PLT test results appear significantly decreased,and the method of ammonium oxalate difference was statistically signifi-cant (t=3.175~3.175,P <0.05);Within 0~60 min 0.9 g/dl NaCl method to detect the PLT differences between the re-sults with the method of ammonium oxalate had no statistical significance (t=0.694~ 1.062,P >0.05).Conclusion ED-TA,citron acid sodium,blood thinners and 0.9 g/dl saline diluting instrument immediately detected PLT PTCP patients were consistent with ammonium oxalate method.Citron acid sodium within 30 minutes and blood dilution method in patientswith PTCP PLT detection could achieve ideal effect,but there were still a small amount of PLT gathered and led to a slight drop in PLT.0.9 g/dl saline diluting instrument method with ammonium oxalate within 0~60 minutes method to detect the PLT result had no difference.
