Evaluation of the Method Change from JSCC to IFCC for ALT,AST,GGT and LDH Detection
10.3969/j.issn.1671-7414.2015.02.034
- VernacularTitle:ALT，AST，GGT 和 LDH 检测JSCC 法变更 IFCC 法性能验证
- Author:
Yachun LU
;
Fen NING
;
Tingting LI
;
Kaiquan HUANG
- Publication Type:Journal Article
- Keywords:
IFCC;
JSCC;
performance verification;
precision;
linear range;
AMR;
regression analysis
- From:
Journal of Modern Laboratory Medicine
2015;(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Abstact:Objective To evaluate the method change from JSCC to IFCC for ALT,AST,GGT and LDH Detection.Methods The accuracy,precision,linearity and reportable range of the new detection method for ALT,AST,GGT and LDH,and the comparison analysis on the two different reagents were evaluated.Results All the accuracy bias of the testing items were within the required 1/2TEa,and all the within-run precision and between run precision were within the required 1/4TEa and 1/3TEa respectively.The linear verification results got the regression equation of the theoretical and measured valuesY =aX+b,in which a was within the range of 0.97~1.03,b was within an acceptable range.The reportable range verification re-sults showed that after the samples being diluted by different proportions,the measured/expected values were all between 90% and 110%,indicating that within a certain range of sample dilution the test esults were reliable.The comparison results showed the R 2 closed to 1.Conclusion The evaluation of the method change for ALT,AST,GGT and LDH detection met the basic requirements of the experiments in clinical diagnosis.
