Study on the Quinolone Antibiotic Resistance and Its Relative Genes in Campylobacter Jejuni
10.3969/j.issn.1671-7414.2015.02.008
- VernacularTitle:空肠弯曲杆菌中喹诺酮类抗生素耐药基因检测与耐药性研究
- Author:
Yunpeng CHEN
;
Wen LIN
;
Jianping DENG
- Publication Type:Journal Article
- Keywords:
real-time fluorescence PCR;
campylobacter jejuni;
drug resistance gene;
quinolone antibiotics
- From:
Journal of Modern Laboratory Medicine
2015;(2):27-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a real-time fluorescence PCR method to detect the drug resistance genes of pathogenic Campylobacter jejunum in human stool samples,and investigate the relationship between quinoloneantibiotic resistance and the related genes in Campylobacter jejuni .Methods According to the gyrA and gyrB gene sequences that related with the fluoroquinolone resistance in Campylobacter jejuni ,the primers of the PCR method was designed and synthesized.A rapid real-time fluorescence PCR method to detect the drug resistance genes in Campylobacter jejuni samples was established,and the optimum reaction system and conditions were screened through an optimized approach.The developed method was com-pared with the classical drug susceptibility assay.Results It was found in the compared results that,there were 8 inconsis-tent strains of Campylobacter jejuni ,2 of the 8 strains were drug sensitive but contented the drug resistance gene,while 6 strains were drug resistant but had no drug resistant gene.Conclusion The established method can be applied to detect the drug resistance relative genes of gyrA and gyrB in Campylobacter jejuni .There was some correlation between the drug re-sistance representation and its genotype,but this point requires further studies.
