Comparison Analysis of HLA-B27 Detected by PCR-SBT and IMS-ELISA in AS Patients
10.3969/j.issn.1671-7414.2015.02.005
- VernacularTitle:PCR-SBT 与 IMS-ELISA 法在强直性脊柱炎患者 HLA-B27检测中的比较
- Author:
Lingdi SUN
;
Chuanzhong MEI
;
Pingjun WANG
;
Xiaoqian WU
;
Xianan SHAO
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction sequence-based typing(PCR-SBT);
immunomagnetic separation and enzyme-linked im-munosorbent assay(IMS-ELISA);
human leukocyte antigen B27(HLA B27);
ankylosing spondylitis(AS)
- From:
Journal of Modern Laboratory Medicine
2015;(2):16-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis (AS)pa-tients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases.Chisquare test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to evaluate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of suspected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83%(55/120)and 37.50% (45/120),respectively.There was statistical difference between the two methods in the HLA-B27 detection (χ2 =59.455,P =0.000).The sensibility and spe-cificity of PCR-SBT were 96.36% and 96.92%,respectively.While the sensibility and the specificity of IMS-ELISA were 69.09% and 89.23%,respectively.Area under the curve of two methods were 0.966 and 0.792,respectively.Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher in AS diag-nosis,that is to say,PCR-SBT is better in HLA-B27 detection and AS diagnosis.
