Value of Human Rhinovirus Detected in Cerebrospinal Fluid of Children with Viral Encephalitis
10.3969/j.issn.1671-7414.2015.02.004
- VernacularTitle:病毒性脑炎患儿脑脊液人类鼻病毒检测的价值
- Author:
Jun GAO
;
Qiong WANG
;
Guangyu LIN
;
Jieling CHEN
;
Xuedong LU
- Publication Type:Journal Article
- Keywords:
human rhinovirus;
viral encephalitis;
children;
nested reverse transcription-polymerase chain reaction
- From:
Journal of Modern Laboratory Medicine
2015;(2):11-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the pathogen of viral encephalitis(VE)in children,and establish a rapid and specific method for detecting human rhinovirus(HRV),and investigate the correlation between HRV and viral encephalitis(VE). Methods 169 CSF specimens were collected from children with convulsions and fever,who were admitted to the pediatric intensive care unit (PICU)of Second Affiliated Hospital of Shantou University Medical College between January 2012 and December 2012.Nested RT-PCR was used to detected HRV in CSF specimens,and the positive PCR products were se-quenced,then analyzed and constructed the phylogenetic tree by software.Results 39 (23.1%)out of 169 samples were HRV positive.Among them,148(87.6%)children were below 5 years old.The detection rate of HRV increases from July to September,and reached its highest point in September.Sequence analyzed showed that the 39 HRV positive specimens inclu-ding 18(46.1%,18/39)positive for HRV-A,7(17.9%,7/39)positive for HRV-B,14(35.9%,14/39)positive for HRV-C. There were 8 out of 28 VE cases were detected in HRV,including 3(50%,3/6)positive for HRV-C.Conclusion HRV could be detected in CSF specimens by nested RT-PCR,including three types of HRV,combined with clinical symptoms con-sidered that HRV may be one of the VE pathogen.
