Study on the Differential Expression of miRNA Screening and MiR-2 2 1 Regulates Apoptosis by Targeting PUMA Gene in Lung Cancer
10.3969/j.issn.1671-7414.2015.01.005
- VernacularTitle:肺癌组织中调控PUMA基因的miRNA筛查和MiR-221表达差异研究
- Author:
Bo JIANG
;
Yuming WANG
;
Yong DUAN
- Publication Type:Journal Article
- Keywords:
lung cancer;
PUMA;
MiR-221
- From:
Journal of Modern Laboratory Medicine
2015;(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the identification of MiR-221 regulates apoptosis by targeting PUMA in lung cancer. Methods The express levels of miRNAs that collected from lung cancer tissue and lung benign lesion were tested by the particular kid they made,analyzed the correlation between individual miRNA.Results 9 6 possible regulated PUMA related miRNAs had been screened through the bioinformatics database,then customized it into the special kit to test the expression with real time fluorescent PCR.The results showed that 10 types miRNAs were up expression in lung cancer tissue,miR-NA221 were selected for deep analyze.Results with rank sum test showed statistical significance (P<0.05)in lung cancer and para cancer tissue.There were statistically significant differences in the expression between metastasis and nonmetastasis (P<0.05),but had nothing to do with the pathological types.Conclusion Real time fluorescent PCR test showed several types of PUMA related miRNAs up-expressed in tissue lung,which indicate miRNA have relationship with PUMA in gene express regulation and cancer generation and development.Pression situation and clinic pathological parameters.Expression of miRNA-221 in lung cancer was associated with tumor metastasis.
