Differences of Nuc-mecA Gene Carriage in the Noses among Different Altitudes in High Humidity District
10.3969/j.issn.1671-7414.2015.01.004
- VernacularTitle:高湿地区不同海拔人群鼻腔nuc-mecA基因定植研究
- Author:
Juan HU
;
Huaiyu XUE
;
Yonghe HU
- Publication Type:Journal Article
- Keywords:
high humidity;
plateau;
nuc-mecA gene;
planting;
real-time fluorescent PCR
- From:
Journal of Modern Laboratory Medicine
2015;(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the carriage of nuc-mecA gene among different altitudes in high humidity district,provid-ed guiding data for prevention of staphylococcus aureus and drug-resistant bacteria,standardizing the usage for antibiotics. Methods The nose swabs were collected in different altitudes:1 000 m,1 200 m and 1 400 m,nuc-mecAgene was confirmed by multi-channel real-time PCR.Results The carrier of nuc gene in the noses were 4.878%,2.899% and 7.143%,in 1 000 m,1 200 m and 1 400 m respectively,and there were no statistical significant among the altitudes (P>0.05).The carrier of mecA gene were 14.634%,31.884% and 41.837% in the 1 000 m,1 200 m and 1 400 m respectively,the difference showed statistical significe (P<0.05).The carrying rate of mecA gene showed statistically significant differences between 1 000 m and 1 200 m (P<0.05),and showed statistically significant differences between 1 000 m and 1 400 m (P<0.01)also.The carrier of nuc-mecA gene were 0%,1.449% and 3.061% in 1 000 m,1 200 m and 1 400 m respectively,there were no statis-tical significant (P>0.05).Conclusion The carrier of mecA gene in noses was increased with the increasing of the altitude. The residents who living at higher altitude should keep the colonization sites of pathogens clean,and needed timely medical when got sick,shouldn’t abuse the antibiotics without authorization.Medical staff should rational use of antibiotic drugs,a-voided overusing of antibiotics and overtreatment.
