Application of Molecular Biology in Investigation of Resistance in Enterococci
10.3969/j.issn.1671-7414.2015.01.002
- VernacularTitle:分子生物学方法在肠球菌耐药性研究中的应用
- Author:
Shan WANG
;
Yuan LU
- Publication Type:Journal Article
- Keywords:
enterococci;
resistance;
molecular biology;
vancomycin-resistant enterococci
- From:
Journal of Modern Laboratory Medicine
2015;(1):4-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to re-sistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent lit-eratures on resistance of Enterococci and a systemic analysis was conducted.Results Common detecting methods include polymerase chain reaction (PCR),pulsed field gel electrophoresis (PFGE),multilocus sequence typing (MLST)and South-ern blot.There also exist less widely-used methods such as pyrosequencing and genechip technique,which may prove effi-cient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.
