Study on the Related Molecular and Cytogenetic Abnormalities in Multiple Myeloma

VernacularTitle:多发性骨髓瘤分子细胞遗传学异常相关性研究
Author: Peiqin SHI ; Wenting WANG ; Xuetao ZHU ; Haiqing WANG ; Yan YANG
Publication Type:Journal Article
Keywords: multiple myeloma; chromosomal abnormalities; IgH gene
From: Journal of Modern Laboratory Medicine 2014;(5):77-80
CountryChina
Language:Chinese
Abstract: Objective To explore the molecular and cytogenetic abnormalities in multiple myeloma (MM).Methods The la-boratory data of bone marrow smears were retrospectively analyzed in 61 patients of MM.24 hours short-term culture of bone marrow and R banding technology were performed in 31 patients.Among these patients,10 cases were selected for de-tecting the IgH gene expression by the interval FISH method.Results The proportions of myeloma cells were 0.19~0.94 in bone marrow smears of 61 patients.In 31 patients,25 patients had enough metaphases for analysis,in which 19 cases (71.3%)had abnormal clones,8q24,11q13,13q14 and 17p13 were important structural abnormalities,where 14q32 rear-rangement was the most characteristic abnormal structure,6 patients were detected IgH gene rearrangement.Conclusion Bone marrow smear combined with other laboratory examinations could make the diagnosis of MM,chromosomal abnormali-ties may help to explore the pathogenesis of MM,and provide a theoretical basis for the early diagnosis,treatment and prog-nosis of this disease.