Increased TRAIL Expression in Peripheral Blood Mononuclear Cells from Patients with Primary Immune Thrombocytopenia and Its Clinical Significance
10.3969/j.issn.1671-7414.2014.05.009
- VernacularTitle:TRAIL在原发免疫性血小板减少症患者外周血单个核细胞中的表达及临床意义
- Author:
Xin YE
;
Lei ZHANG
;
Mingli GU
;
Weiwei ZHANG
;
Anmei DENG
;
Baohua QIAN
- Publication Type:Journal Article
- Keywords:
primary immune thrombocytopenia;
peripheral blood mononuclear cells;
TRAIL
- From:
Journal of Modern Laboratory Medicine
2014;(5):46-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore TRAIL expression in patients with primary immune thrombocytopenia (ITP)and its role in the pathogenesis.Methods Peripheral Blood Mononuclear Cells (PBMCs)from twenty-eight patients with ITP and thirty healthy controls were obtained.TRAIL expression was determined using Real-time Quantitative Polymerase Chain Reaction (RT-PCR).Serum TNF-α,IFN-γ,IL-4 and IL-10 were detected using ELISA.Associations between TRAIL expression and clinical parameters were assessed.Results Compared to healthy controls,TRAIL expression was significantly increased in PBMC from patients with ITP (2.80±0.43 vs 1.00±0.24,t=19.72,P<0.001).Compared to the healthy controls,ex-pression of IFN-γand TNF-αfrom ITP patients were increased (52.43±11.04 pg/ml vs 23.26±8.15 pg/ml;69.14± 10.89 pg/ml vs 36.14±13.17 pg/ml,t=10.36~11.50,P<0.001)and expression of IL-10 were decreased (11.18±6.13 pg/ml vs 33.28±9.85 pg/ml,t=10.17,P<0.001)and there was no significant difference in IL-4 expression (35.75± 12.52 pg/ml vs 40.16±14.26 pg/ml,t=1.25,P>0.05).Furthermore,TRAIL expression was positively correlated with serum IFN-γ,TNF-αlevel (r=0.432,0.541,P<0.05),and negatively correlated with IL-10 and PLT (r=-0.424,-0.553,P<0.05).Conclusion Rur results suggest that TRAIL may be involved in the pathogenesis of ITP.
