Increased Expression of T Cell Immunoglobulin and Mucin-3 in Peripheral Blood Mononuclear Cells from Patients with Primary Sj¨ogren’s Syndrome and Its Clinical Significance
10.3969/j.issn.1671-7414.2014.05.004
- VernacularTitle:原发性舍格伦综合征患者外周血单个核细胞中TIM-3表达增高及意义
- Author:
Wei KONG
;
Lei ZHANG
;
Mingli GU
;
Weiwei ZHANG
;
Anmei DENG
;
Sunxiao CHEN
- Publication Type:Journal Article
- Keywords:
Sj¨ogren’s syndrome;
TIM-3;
RT-PCR
- From:
Journal of Modern Laboratory Medicine
2014;(5):31-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of T cell immunoglobulin and mucin-3(TIM-3)in peripheral blood mononu-clear cells from patients with primary Sj¨ogren’s syndrome(pSS)and its clinical significance.Methods Enrolled 33 pSS pa-tients from Shanghai Changzheng Hospital and Changhai Hospital in October 2012 and July 2013.The relative expression of TIM-3 in the peripheral blood mononuclear cells(PBMC)in 33 patients and 33 healthy individuals was detected by RT-PCR. The association between TIM-3 mRNA level and clinical characteristics were analyzed,for example,serum RF,CRP and anti-SSA/SSB antibody.1 2 of the pSS patients were followed up and their TIM-3 mRNA level was determined after glucocorti-coid treatment for 2 months.Results The expression of TIM-3 in PBMCs from patients with pSS was increased significantly than that in healthy individuals (0.55±0.12 vs 0.13±0.10,t=15.44,P<0.000 1),but its expression between anti-SSA/anti-SSB positive and negative patients were not statistical difference (0.45±0.21 vs 0.54±0.31;0.46±0.15 vs 0.51± 0.24).The TIM-3 mRNA expression in pSS patients was positively correlated with serum RF and CRP (R=0.558 5,R=0.414 7,P>0.05),but was not associated the anti-SSA/SSB antibody (P=0.298 2).TIM-3 mRNA level was decreased in the patients who were treated with glucocorticoids for 2 months (0.62±0.10 vs 0.38±0.13,t=5.07,P<0.05).Conclusion TIM-3 may play a role in the pathogenesis of pSS and it may be regarded as a biomarker to pSS.
