Establishment of the Quantitative Immunohistochemical Examination of DHBcAg in Duck Liver
10.3969/j.issn.1671-7414.2014.05.002
- VernacularTitle:鸭肝组织中DHBcAg表达水平定量免疫组织化学方法的建立
- Author:
Yawen WANG
;
Xi LIU
;
Lingyun HUI
;
Hui GONG
;
Lin ZHANG
;
Lieting MA
;
Shuiping HAN
;
Quanying WANG
;
Guangxiao YANG
;
Zhengwen LIU
- Publication Type:Journal Article
- Keywords:
duck hepatitis B virus core antigen (DHBcAg);
immunohistochemistry;
Image-Pro Plus 6.0 software;
quantitative assay
- From:
Journal of Modern Laboratory Medicine
2014;(5):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a quantitative immunohistochemistry assay for duck hepatitis B virus core antigen (DHB-cAg)in duck liver tissue.Methods By comparison with no repair antigen and repair antigen with high pressure,microwave and trypsin,the best solution of antigen retrieval was determined.By optimizing the parameter of image acquisition and de-ducting blank area,mean density of yellow areas was calculated using Image-Pro Plus 6.0 software.Using the assay devel-oped to determine the level of DHBcAg in liver tissue from duck infected by DHBV,anti-DHBV activity of DHBcMAb-TAT PTD conj ugate was examined.Results SABC method with no repair antigen was selected,which was better than other methods.DHBcAg expression in duck liver tissue could be objectively and accurately quantified by setting Image-Pro Plus 6.0 software parameters and calculating mean density of yellow areas.By comparison with the differences between mean densityat baseline of treatment and end of treatment,it was showed that DHBcMAb-TATPTD conjugate treatment dose-de-pendently reduced the levels of DHBcAg in liver tissue,which show that the assay developed could effectively evaluate the anti-DHBV activity of agent.Conclusion The immunohistochemistry assay developed in this study can objectively and accu-rately evaluate the level of DHBcAg in duck liver tissue.
