Bioinformatic analysis of small RNA expression during chondrogenesis in rats
10.7652/jdyxb201504007
- VernacularTitle:大鼠软骨生成过程表达的小分子 RNA 生物信息学分析
- Author:
Shibin SHAO
;
Zixin MIN
;
Yuanxu GUO
;
Quancheng WANG
;
Mengyao SUN
;
Yan HAN
;
Jian SUN
- Publication Type:Journal Article
- Keywords:
small RNA (sRNA);
bioinformatics;
chondrogenesis;
rat;
Solexa sequencing
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(4):462-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the profiles and function of small RNA (sRNA)gene during chondrogenesis in rats so as to clarify the mechanisms of chondrocytes proliferation and differentiation.Methods All the sRNAs were identified from the female SD rats femoral head cartilages at three time points:at birth,ablactation and maturation,and three sRNA libraries were constructed.The Solexa sequencing and the bioinformatics analysis were employed to be blasted with the genomes of SD rats.Results The perfect match reads in the three libraries were screened out,which were correspondent to the 21 7 921 (41.23%),1 96 650 (38.74%)and 245 436 (41.54%)unique sRNA sequence,respectively.The percentages of 20-24 nt sRNA were 71.94% (d0),72.85% (d21),and 86.39%(d42).Half of clean sequences were 22 nt sRNA.The distribution characteristics of the reads were in line with the high-quality sRNA.More than 62% clean reads were from mature miRNA while the ratios in the three libraries were only 0.69%,0.78% and 0.63%.About 60% of the unique sRNA could not be matched with miRBase20.0 or Rfam9.1.Conclusion The distribution model of miRNA in the three libraries indicates that the miRNAs with different functions or from different sources are involved in the regulation of chondrocytes proliferation and differentiation in bone development and formation.
