Effect of recombinant murine erythropoietinon injected into mice with multiple myeloma on their immune function

VernacularTitle:多发性骨髓瘤小鼠注射重组鼠源促红细胞生成素后对免疫功能的影响
Author: Hailing TANG ; Limei FAN
Publication Type:Journal Article
Keywords: multiple myeloma; recombinant human erythropoietin; immune function
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2015;(4):487-490
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of recombinant human erythropoietin on immune function in mice with multiple myeloma.Methods Multiple myeloma model mice were divided randomly into control group and treatment groups (low,middle and high doses).The rats in treatment groups were injected with recombinant human erythropoietin 100 μL (2.5,5.0 and 10 mg/kg)via the tail vein for 30 days,and the rats in control group were injected isotonic saline solution of the same volume instead.After the experiment,the colorimetric MTT assay,neutral red method and ELISA kit were used to evaluate the proliferation of B and T lymphocytes,the phagocytic ability of macrophages and the content of serum TNF-α.Results Compared with those in control group,different doses of recombinant human erythropoietin could significantly increase the proliferation of lymphocytes,increase the phagocytosis of macrophages and improve the production of serum TNF-α(P < 0.05 ). Conclusion Recombinant human erythropoietin can significantly improve immunoregulation of mice with multiple myeloma.