Effects of Sufentanil in inhibiting Hep3B cell viability and promoting cell apoptosis in liver cancer
10.7652/jdyxb201504010
- VernacularTitle:舒芬太尼抑制肝癌 Hep3B 细胞活性及促进凋亡作用
- Author:
Wei GAO
;
Xiaoqi ZHANG
;
Guixia JING
;
Pingyi SONG
;
Kejun NAN
;
Baoyong SHA
- Publication Type:Journal Article
- Keywords:
Sufentanil;
Hep3B cell;
cell viability;
apoptosis;
survivin;
caspase-3
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(4):479-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of Sufentanil on Hep3B cell viability in liver cancer.Methods Sufentanil of different concentrations (0.01,0.1,1,5,10,1 5 μmol/L)was used to treat Hep3B cells.The changes of cell cycle,apoptosis and protein expression were detected to explore the potential mechanisms by MTT method,flow cytometry and Western blot,respectively.Results Reduced viability of Hep3B cells,arrested cell cycle in the G0/G1 phase,decreased expression of survivin protein,and increased expression of caspase-3 protein were observed with the increase of Sufentanil concentration. Conclusion Sufentanil inhibited the viability of Hep3B cells through affecting cell cycle,promoting cell apoptosis and changing protein expressions of survivin and caspase-3.
